Hydrocortisone in low doses has a protective effect on hemorrhagic shock in rats

Authors

  • Marcelo Luiz Couto Tavares Universidade do Estado do Pará
  • Andrew Moraes Monteiro Universidade do Estado do Pará
  • Daniel Haber Feijó Universidade do Estado do Pará
  • Carolina Ribeiro Mainardi Universidade do Estado do Pará
  • Rubens Fernando Gonçalves Ribeiro Junior Universidade do Estado do Pará
  • Daniela Vale Dias Universidade Federal do Pará
  • Marcus Vinicius Henriques Brito Universidade do Estado do Pará

DOI:

https://doi.org/10.4322/prmj.2017.008

Keywords:

hydrocortisone, hypertonic saline solution, hemorrhagic shock, ischemia, reperfusion, inflammation, blood gas analysis, rats

Abstract

Purpose: To evaluate the hydrocortisone low-dose effects in hemorrhagic shock in rats.Methods: Twenty male Wistar rats were randomly distributed into 04 groups: Sham Group (GS); Shock group (CG), animals subjected to hemorrhagic shock; Group Hypertonic Saline 7.5% (GSH 7.5%), with volume replacement of saline solution at 7.5% after shock; Hydrocortisone Group (GH), animals received hydrocortisone after shock. Arterial blood gas samples and variations of the mean arterial blood pressure (MAP) were analyzed before the shock induction and after treatments. Intestinal samples were collected for histological analysis. Results: MAP was statistical significantly increased after treatment of the shock in GSH 7,5% (p=0.0001) and GH (p=0.0058) when compared to the CG. Regarding the arterial blood gas analysis, hydrocortisone increased pH of arterial blood in comparison to the GC (P=0.0372), lowered PaCO2 (p=0.0008), maintained higher levels of bicarbonate ions (p=0.0395) and lower levels of lactate (p=0.002). GH (p=0.02), as GSH7.5% (p=0.0147), showed less histological damage than the GC.Conclusion: Low dosages of hydrocortisone had protective effects against hemorrhagic shock in rats.

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Author Biographies

Marcelo Luiz Couto Tavares, Universidade do Estado do Pará

Médico anestesiologista com título de especialista em Anestesiologia emitido pela Sociedade Brasileira de Anestesiologia; mestre pelo Programa de Pós-graduação em Cirurgia e Pesquisa Experimental da Universidade do Estado do Pará (UEPA).

Andrew Moraes Monteiro, Universidade do Estado do Pará

Estudante de graduação da Faculdade de Medicina da Universidade do Estado do Pará (UEPA).

Daniel Haber Feijó, Universidade do Estado do Pará

Estudante de graduação da Faculdade de Medicina da Universidade do Estado do Pará (UEPA).

Carolina Ribeiro Mainardi, Universidade do Estado do Pará

Estudante de graduação da Faculdade de Medicina da Universidade do Estado do Pará (UEPA).

Rubens Fernando Gonçalves Ribeiro Junior, Universidade do Estado do Pará

Estudante de graduação da Faculdade de Medicina da Universidade do Estado do Pará (UEPA).

Daniela Vale Dias, Universidade Federal do Pará

Estudante da graduação da Faculdade de Medicina da Universidade Federal do Pará (UFPA).

Marcus Vinicius Henriques Brito, Universidade do Estado do Pará

PhD; professor doutor e professor titular da disciplina de Técnica Operatória, Cirurgia Experimental e Anestesiologia da Universidade do Estado do Pará (UEPA).

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Published

2017-07-30

How to Cite

Tavares, M. L. C., Monteiro, A. M., Feijó, D. H., Mainardi, C. R., Ribeiro Junior, R. F. G., Dias, D. V., & Brito, M. V. H. (2017). Hydrocortisone in low doses has a protective effect on hemorrhagic shock in rats. Pará Research Medical Journal, 1(1), 1–8. https://doi.org/10.4322/prmj.2017.008

Issue

Vol. 1 No. 1 (2017)

Section

Research Article